MacConkey Agar is nice and good product, MacConkey agar is a culture medium which is designed to grow Gram-negative bacteria and stain them for lactose fermentation.
Making procedures:
1 .Let peptone, bile salts and sodium chloride dissolve in 400mL distilled water, the correction pH7.2. The agar by adding 600mL
Heat it in order to dissolve. Merge the two fluids, packing in the flask, 121 ℃ 15min spare autoclave.
2 temporary use when heated to melt the agar, lactose added hot and cold to 50 ~ 55 ℃ , adding crystal violet and neutral red solution, shake well and pour plate.
Note: The crystal violet and neutral red solution should autoclave.
Read more: http://www.cellular-products.com/
No comments:
Post a Comment