MacConkey agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. Acting as a visual pH indicator, the agar distinguishes those Gram-negative bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-).
MacConkey agar is used for isolation and culture of enteric pathogens and non-fermenting bacteria identification.
[Preparation Method]
Peptone 20g, NaCl 5g, bile salts (pigs, cattle, sheep) 5g, lactose 10g, agar 15 ~ 20g, 1% neutral red solution, 5ml, distilled water 1L.
let The above ingredients (except the neutral IR) to weight, and then pour it into the water, heated to dissolve, corrected to pH 7.2 by adding neutral red solution, packaging and sterilization115 ℃20min, cooled to about50 ℃when the pour plate
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