Thursday 29 December 2011

How to use Ornithin Decarboxlase Medium

Ornithin Decarboxlase Medium presents in all nucleated cells and is the rate-limiting enzyme for synthesis of polyamines.It is used for the cultivation and differentiation of bacteria based on their ability to decarboxylate the amino acid. Bacteria that decaboxylate ornithine turn  the medium turbid purple.
Ornithin Decarboxlase Medium is nice Biochemical medium.
Usages:
Ornithin Decarboxlase Medium is used for the identification of intestinal species with the ability to produce amino acid decarboxylase.
Original rationale:
Peptone and yeast extract powder to provide nitrogen, vitamins and growth factors; glucose to fermentable sugars; bromocresol purple as pH indicator, acid yellow, purple alkaline; in basic medium by adding lysine, arginine acid and ornithine test whether the bacteria produce the enzyme than the substrate.

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