Wednesday 30 November 2011

Supply XceI (NspI)

Product name: XceI (NspI)    
Specification: 1000U/200U
Source: Xanthomonas campestris Ast 40–024
Storage Buffer:10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Description: After 50-fold overdigestion (3u/ug DNA x 17 hours) with XceI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.32uM. More than 95% of these can be recut.One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
Storage:-20°C

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