Friday, 30 December 2011

How to get Sodium benzoate

Sodium benzoate  is commonly used food preservative, to prevent deterioration sour, the effect of extending shelf life, are widely used in the world. However, in recent years, concerns about its toxicity, makes it of limited in application.
Sodium benzoate is the sodium salt of benzoic acid.
Sodium benzoate, also known as benzoic acid, it is also known as sodium benzoate sodium benzoin. Benzoic acid is insoluble in water at room temperature in air (especially the hot air) in the micro-volatile, hygroscopic.
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Thursday, 29 December 2011

How to use Ornithin Decarboxlase Medium

Ornithin Decarboxlase Medium presents in all nucleated cells and is the rate-limiting enzyme for synthesis of polyamines.It is used for the cultivation and differentiation of bacteria based on their ability to decarboxylate the amino acid. Bacteria that decaboxylate ornithine turn  the medium turbid purple.
Ornithin Decarboxlase Medium is nice Biochemical medium.
Usages:
Ornithin Decarboxlase Medium is used for the identification of intestinal species with the ability to produce amino acid decarboxylase.
Original rationale:
Peptone and yeast extract powder to provide nitrogen, vitamins and growth factors; glucose to fermentable sugars; bromocresol purple as pH indicator, acid yellow, purple alkaline; in basic medium by adding lysine, arginine acid and ornithine test whether the bacteria produce the enzyme than the substrate.

Wednesday, 28 December 2011

Where to get Chloroform


Chloroform is organic chemicals, it is mainly used to produce Freon (F-21, F-22, F-23), dyes and drugs, in medicinefield,it is  used as anesthetic.also Can be used as antibiotics, spices, oils, resins, rubber solvent and extractant.
Chloroform is colorless transparent liquid. It has a special smel and Sweet taste.
Several million tons are produced annually as a precursor to Teflon and refrigerants, but the latter application is being phased out.
Chloroform once was a popular anesthetic; its vapor depresses the central nervous system of a patient, allowing a doctor to perform various otherwise painful procedures.
Grade:Biotechnology grade
CAS: 67-66-3
Molecular Formula: CHCl3
Molecular Weight: 119.38
Specification: 950ml
Danger:Yes
Storage Condition: Room temperature
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Tuesday, 27 December 2011

What is Insulin from bovine pancreas

Insulin from bovine pancreas is extracted from bovine pancreas; the molecular structure has three different amino acids with human insulin, less effective and prone to allergies or insulin resistance. The only advantage of animal insulin is cheap. Patients can easily afford.
Insulin from bovine pancreas is peptide.Bovine insulin is a protein molecule.
Medical functions:
1. Anti-inflammatory effect
2. Anti-atherosclerosis effect
3. Anti-platelet aggregation
4. Treatment of osteoarthritis
5. Treatment of mental illness

Sunday, 25 December 2011

MacConkey agar for your choice


MacConkey agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. Acting as a visual pH indicator, the agar distinguishes those Gram-negative bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-).
MacConkey agar is used for isolation and culture of enteric pathogens and non-fermenting bacteria identification.
[Preparation Method]
Peptone 20g, NaCl 5g, bile salts (pigs, cattle, sheep) 5g, lactose 10g, agar 15 ~ 20g, 1% neutral red solution, 5ml, distilled water 1L.
let The above ingredients (except the neutral IR) to weight, and then pour it into the water, heated to dissolve, corrected to pH 7.2 by adding neutral red solution, packaging and sterilization115 ℃20min, cooled to about50 ℃when the pour plate
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Thursday, 22 December 2011

The function of Taq DNA Polymerase


Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'=>3' synthesis of DNA, has no detectable 3'=>5' exonuclease (proofreading) activity and possesses low 5'=>3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.
Taq DNA polymerase (recombinant) is cell with a cloned pol gene from Thermus aquaticus YT1.
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Wednesday, 21 December 2011

The benefits of TaqI

TaqI is cellular-product which is produced by our company.
After 50-fold overdigestion (3u/ug DNA x 17 hours) with TaqI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.2uM. More than 95% of these can be recut.
TaqI does not cut TCGm6A. Blocked by overlapping Dam methylation.One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dam-in 1 hour at 65°C in 50ul of assay buffer.
Storage:-20°C
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